immunogenicity of bhk-rabies vaccine in cattle

in this study, the immunogenicity of a rabies vaccine that was used in one of the isfahan cattle farms is reported. this vaccine was produced by replication of the pasteur virus (pv) strain on bhk-21 monolayer cell culture. the virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. thus, the obtained vaccine was tested on mice and a satisfactory immune response was observed. thereafter, the vaccine was tested on 34 cattle divided into 2 groups. each group was received a different dose of the vaccine intramuscularly. sera were taken 2 months after rabies vaccination and were analyzed by the rapid fluorescence focus inhibition test (rffit) to evaluate the titer of the rabies-neutralizing antibody. both groups under study showed a sufficient seroconversion.